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RIKEN CDB
RIKEN CDB

Cassettes

     
 

Cassettes

 
  01. pMC1DT-A cassette A  
  02. pMC1DT-A cassette A(modified)  
  03. pKJ2-Neo  
  04. loxP/PGK-Neo-pA/loxP  
  05. loxP/PGK-Neo/loxP(poly A less)  
  06. loxP/PGK-Neo/loxP(poly A less)〜W Not I〜  
07. frt/PGK-Neo-pA/frt
08. PGK-Neo(poly A less)/DT-A
09. DT-A/loxP/PGK-Neo-pA/loxP  Sequence
10. loxP/PGK-Neo-pA/loxP for Control Vector  Sequence
11. DT-A/Conditional FW  Sequence
12. DT-A/Conditional REV  Sequence
13. DT-A/lox71/LacZ-pA/frt/PGK-Neo/frt/loxP/pA  Sequence
14. lox71/LacZ-pA/frt/PGK-Neo/frt/loxP/pA for Control Vector  Sequence
15. DT-A/AFP-pA/PGK-Neo-pA(*)  Sequence
16. DT-A-pA/loxP/PGK-Neo-pA/loxP  Sequence
17. DT-A-pA/lox71/LacZ-pA/frt/PGK-Neo/frt/loxP/pA  Sequence
18. DT-A-pA/AFP/PGK-Neo-pA(*)  Sequence
19. DT-A-pA/Conditional KO FW  Sequence
20. pENTR Conditional FW for Gateway  Sequence
21. pENTR Conditional REV for Gateway  Sequence
22. pDEST DT-A-pA for Gateway  Sequence
23. pENTR lox71/LacZ-pA/frt/PGK-Neo/frt/loxP/pA for Gateway  Sequence
24. pENTR AFP-pA/PGK-Neo-pA(*) for Gateway  Sequence
25. pENTR loxP/PGK-Neo-pA/loxP for Gateway  Sequence
  *AFP=mutated Aequorea green fluorescent protein
ref. Inouye, S., Ogawa, H.,et al., 1997.A bacterial cloning vector using a mutated Aequorea green fluorescent protein as an indicator.Gene.Apr 21;189(2):159-62
 
     
 
供与核酸
核酸供与体
LacZ遺伝子 大腸菌
薬剤耐性遺伝子(カナマイシン、ネオマイシン、アンピシリン) 大腸菌
DT-A (diphtheria toxin fragment A) コリネバクテリウム属-ジフレテア菌
Cre (P1ファージ組換え酵素遺伝子) バクテリオファージ科-P1ファージ
loxP (組換え酵素認識配列) バクテリオファージ科-P1ファージ
frt 組換え酵素遺伝子 酵母
AFP (mutant Aequorea green fluorescent protein) 有鞘類-オワンクラゲ
(Neoの)PGK promoter, PGK pA signal マウス
(lacZ,AFP,DT-AのpA) Rabbit beta globin pA signal うさぎ
 
     
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