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| 変異マウスの共同開発 | ||
| - 趣旨 | ||
| - お知らせ | ||
| - Knockout Mouse (Vector) | ||
| - Knockout Mouse (ES) | ||
| - Transgenic Mouse | ||
| Joint Development of Mutant Mice with Asian-Pacific Researchers | ||
| - Details | ||
| Tools | ||
| - Protocols | ||
| - Vector Cassettes | ||
| - ES cells developed by LARGE, CDB | ||
| - Methods | ||
| Mutant Mice Generated in Center for Developmental Biology (CDB) | ||
| - KO Mice | ||
| - TG Mice | ||
| Tools for Live imaging | ||
| - Reporter Mice | ||
| - Expression Vectors | ||
| ユニット紹介/About LARGE | ||
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Knockout Mouse |
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１）ターゲティングベクターの構築 |
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| 01. ノックアウトマウスと設計図 | Download PDF | | |||
| 02. Targeting VectorとControl Vecotrの作成｜Download PDF | | |||
| 03. PCRによるHomology Armの増幅｜Download PDF | | |||
| 04. PCRによるスクリーニングの条件検討 ｜Download PDF | | |||
| 05. ランダムインテグレーション検出 ｜Download PDF | | |||
| 06. ゲノム抽出〜サザン解析 ｜Download PDF | | |||
| 07. PCRによるGenotypingの条件検討とテールからのPCR ｜Download PDF | | |||
| 08. サザンに使う制限酵素 ｜Download PDF | | |||
| 09. PCRによるloxP配列挿入の確認 ｜Download PDF | | |||
| 2) 設計図のPowerPoint | |||
| 01.DT-ApA/LacZ/Neo｜Download ppt | | |||
| 02.DT-ApA/Neo｜Download ppt | | |||
| 03.DT-ApA/Conditional KO FW｜Download ppt | | |||
3）Targeting Vector Construction (English) |
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| 01. Vector construction protocol｜Download PDF | | |||
4）References |
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| 01. ang is a novel gene expressed in early neuroectoderm, but its null mutant exhibits no obvious phenotype. ｜Download PDF | | |||
| 02. Homologous recombination at c-fyn locus of mouse embryonic stem cells with diphtheria toxin A fragment gene in negative selection. ｜Download PDF | | |||
| 03. A novel negative selection for homologous recombinants using diphtheria toxin A fragment gene. ｜Download PDF | | |||
| 04. A novel ES cell line, TT2, with high germline-differentiating potency. ｜Download PDF | | |||
05. TT2細胞とマウスの毛色の関係 ｜Download PDF | |
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Transgenic Mouse |
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１）トランスジーン調製 |
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| 01.注入用DNA溶液調製の手引き ｜Download PDF | | |||
| 02.よくある質問｜Download PDF｜ | |||
２）References |
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| Brinster RL, Chen HY, Trumbauer
ME, Yagle MK, Palmiter RD. Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs. Proc. Nail. Acad. Sci. USA 82 4438-4442, July (1985) |
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| Yang XW, Model P, Heintz N. Homologous recombination based modification in Esherichia coli and germline transmission in transgenic mice of a bacterial artificial chromsome.Nature biotechnology 15 859-865 (1997) |
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| Gong S, Zheng C, Doughty ML, Losos K, Didkovsky N, Schambra UB,
Nowak NJ,Joyner A, Leblanc G, Hatten ME, Heintz N. A gene expression atlas of the central nervous system based on bacterial artificial chromosomes.Nature. 425 917-925. (2003) |
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| Gong S, Yang XW, Li C, Heintz N. Highly Efficient Modification of Bacterial Artificial Chromosomes (BACs) Using Novel Shuttle Vectors Containing the R6Kgamma Origin of Replication.Genome Research 12 1992-1998 (2002) |
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| Nagy A, Gertsenstein M, Vintersten K and Behringer R. Manipulating of the Mouse Embryo. A Laboratory Manual. 3rd Edition.Cold Spring Harbor Laboratory Press,2003. | |||
| 発生工学実験マニュアル. トランスジェニックマウスの作り方 勝木元也／編 講談社サイエンティフィク |
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胚操作（準備中） |
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