Laboratory for Evolutionary Regeneration Biology

Kiyokazu AGATA, Ph. D.  

Although all animals possess some ability to regenerate, that capacity varies widely from species to species. The planarian flatworm, a relatively simple organism, exhibits remarkable regenerative ability attributable to a population of pluripotent stem cells capable of giving rise to all cell types in the body. Study of planarian regeneration may enable us to better understand how we rebuild tissues and organs damaged by injury, disease or as part of the natural aging process. The ultimate goals of our laboratory are to understand the mechanisms regulating the planarian stem cell system and to determine what signals are involved in both maintaining these cells' totipotency and in activating them during regeneration.

In recent years, planarian research has benefited from the application of molecular techniques such as whole-mount in situ hybridization and gene-knockdown by RNA interference. One of our laboratory's recent successes has been the identification of the nou-darake gene, a key element in modulating FGF signaling in stem cells to restrict brain tissue to the head region of planarians. These findings may provide valuable insights into the field of regenerative medicine, especially neural regeneration.

The study of planarians may also help to reveal how higher organisms develop complex cell systems, such as the nervous system, during evolution. A bilaterally symmetric animal, the planarian is believed to be the simplest extant taxon to form a distinct head. Although the planarian brain is very simple, it is capable of regulating behaviors in response to a variety of environmental signals. The establishment of EST databases for a variety of species, including the planarian and the fresh water sponge (one of the most primitive forms of multicellular life), promises to provide invaluable resources for the study of evolutionary biology and a basis for comparative analyses of the genomics underlying the shared and distinct features of these organisms.

By focusing on the development of the flatworm brain, we hope to use it as a model to shed light on the contribution of stem cells to the development of increasingly complex cellular systems through evolution, studies which we hope will also help to improve our understanding the role of the stem cell system in evolutionary regeneration biology.