The 17th CDB Meeting October 14-15,2008
Towards Synthesis of Cells-Reconstruction and Design of Cellular Functions-

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Program at a glance (PDF)
Tuesday, October 14
Wednesday, October 15

Poster Program

Tuesday, October 14

10:00 am
Opening Remarks by Hiroki R. Ueda

Session 1 / Construction of Basic Cellular Systems

10:10 am
Mechanisms of ATP-DnaA Assembly and Duplex DNA Unwinding for the Initiation of DNA Replication in Reconstituted Systems
Tsutomu Katayama (Kyushu University, Japan)

10:40 am
Single-molecule Imaging of the Transport-vesicle Formation Mediated by COPII system Reconstituted on a Planner Bilayer System (Tentative)
Hiroyuki Noji (Osaka University, Japan)

11:10 am Coffee Break

Session 2 / New Technologies for Design of Molecules

11:30 am De Novo Computational Design of Protein Structure
Nobuyasu Koga (University of Washington, USA)

12:00 pm Design of Biological Molecules using Molecular Dynamics Simulations
Makoto Taiji (RIKEN, Japan)

12:30 pm Lunch and Poster Session 1

Session 3 / New Technologies for Nano-biosystems

2:30 pm Investigating Cellular Signaling at the Nanometer and Attoliter Scale
Horst Vogel (Swiss Federal Institute of Technology Lausanne (EPFL), Switzerland)

3:00 pm A stochastic single-molecule event triggers phenotype switching in a bacterial cell
Paul J. Choi (Harvard University, USA)

3:30 pm Coffee Break

Session 4: Synthesis of Higher-order Cellular Systems

3:50 pm

KaiC as Timebase for Circadian Clock of Cyanobacteria
Takao Kondo (Nagoya University, Japan)

4:20 pm
Towards Reconstruction of Mammalian Circadian Clocks
Hiroki R. Ueda (RIKEN CDB, Japan)

4:50 pm Reconstituting C. elegans Germ Granules in Mammalian Cells
Asako Sugimoto (RIKEN CDB, Japan)

5:20 pm Coffee Break

Session 5 / Programming Cells

5:40 pm A Spatial Gradient of Cell Adhesion Peptide in a Three-dimensional Scaffold Directs Osteoblastic Differentiation of Mesenchymal Stem Cells*
Masaya Yamamoto (Kyoto University, Japan)

6:00 pm Induction of Pluripotency by Defined Factors
Shinya Yamanaka (Kyoto University, Japan)

6:30 pm Reception

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Wednesday, October 15

Session 6 / Towards Synthesis of Organs

10:00 am
How to determine the size of the embryonic brain: robustness factors for quantitative dorsal-ventral patterning
Yoshiki Sasai (RIKEN CDB, Japan)

10:30 am
From the Bioimaging to the Numerical Analysis
Hideo Yokota (RIKEN, Japan)

11:00 am Coffee Break

Session 7 / Design of Functional Molecular Systems

11:20 am Rheology of Reconstituted Networks of Biopolymers
David Weitz (Harvard University, USA)

11:50 am Population Study of Sizes and Components of Self-Reproducing Giant Multilamellar Vesicles*
Taro Toyota (The University of Tokyo, Japan)

12:10 pm Lunch and Poster Session 2

Session 8 / Design of Proto-type Cells

2:00 pm Cell-free Expression: Application to Gene Network and Synthetic Vesicles
Vincent Noireaux (University of Minnesota, USA)

2:30 pm Construction of Self-Reproducible Synthetic Cell*
Yutetsu Kuruma (The University of Tokyo, Japan)

2:50 pm
In liposome RNA-Protein Self-Replication System
Tetsuya Yomo (Osaka University, Japan)

3:20 pm
Coffee Break

Session 9 / Towards Design of Minimal Cells

3:40 pm The Open Cell Project: Complexity Reduction of Mesoplasma florum
Thomas F. Knight (MIT, USA)

4:10 pm The quest for a minimal cell: a synthetic genomics approach
Clyde A. Hutchison III (J. Craig Venter Institute, USA)

4:40 pm Closing Remarks by Hiroyuki Noji

* Selected talks

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Poster Session

P01 Synthesis of Higher-Ordered Cellular Systems - Mechanisms for Establishing and Maintaining Cell Identity in the C. elegans Embryo
Nareg Djabrayan (University of California, Santana Barbara, USA)

P02 Comparison of Bone Marrow Mesenchymal Stem Cell and Peritoneal Stem Cell : For Purposes of Surface Markers
Tahereh Foroutan (Tarbiat Moallem Univesity, Iran)

P03 Melanopsin-Expressing Retinal Ganglion Cells Serve as the Site for the Divergence of Image-Forming and Non-Image Forming Photoresponses
Megumi Hatori (The Salk Institute for Biological Studies, USA)

P04 Importance of Translation-replication Balance for Efficient Replication of Genetic Information
Norikazu Ichihashi (Osaka University, Japan)

P05 Platform for Controlling Micro-Emulsions as a Model of Growth and Division Cycle of the Cell
Tetsuo Ichii (ERATO, JST, Japan)

P06 Identification of Molecular States and Atomic Interactions by Potential Energy Principal Component Analysis Method
Yohei M. Koyama (RIKEN Center for Developmental Biology, Japan)

P07 Construction of Self-Reproducible Synthetic Cell
Yutetsu Kuruma (The University of Tokyo, Japan)

P08 Rapid Fabrication of Cell Spheroids by Using Micro-patterned Chamber
Takuya Matsumoto (Osaka University, Japan)

P09 Biochemical Approaches for Temperature-insensitivity of the Circadian Clock toward Reconstitution of the Mammalian Circadian Clock
Masato Nakajima (RIKEN Center for Developmental Biology, Japan)

P10 Levitated Drop Reconstitution of Hydrophobic Protein Array in Lipid Membrane Bilayer Towards Crystallization of Membrane Proteins and Assembly of Cellular Coating
Lukas Pichl (RIKEN Wako Campus, Japan)

P11 The Synthesis of Artificial Stochastic Biochemical Pathways from Generic Designs
Marc Riedel (University of Minnesota, USA)

P12 Reconstitution of tRNA in a Cell-free Protein Synthesis System
Yoshihiro Shimizu (The University of Tokyo, Japan)

P13 Mathematical Model and Real-world Model for the Synthesis of Cell Functions Including Nonequilibricity of Biomembrane and On/Off Characteristics of Giant DNA
Masahiro Takinoue (Kyoto University, Japan)

P14 Population Study of Sizes and Components of Self-Reproducing Giant Multilamellar Vesicles
Taro Toyota (Chiba University, Japan)


P16 Electroformed Giant Vesicles from Micropatterned Lipid Films
Andrew Utada (The University of Tokyo, Japan)

P17 Characterisation of a Torque Component in the Kinesin Power Stroke
Junichiro Yajima (Gakushuin University, Japan)

P18 A Spatial Gradient of Cell Adhesion Peptide in a Three-dimensional Scaffold Directs Osteoblastic Differentiation of Mesenchymal Stem Cells
Masaya Yamamoto (Kyoto University, Japan)

P19 Algebraic Relations Between Cell-type Diversity and Cell-type Ratio over Lindenmayer Cell-chain Model
Hiroshi Yoshida (Kyushu University, Japan)

P20 Lipid Bilayer Enclosed Microchamber Array for Studying Cellular Transmembrane Responses
Satoko Yoshizawa (The Univercity of Tokyo, Japan)

P21 Spontaneous Motion of Oil/Water Interface Responding to Specific Cation, Ca2+ or Fe3+
Akihisa Shioi (Doshisha University, Japan)

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