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Category CDBセミナー
Date and Time 2013-02-22 15:00 - 16:00
Venue Seminar Room A7F
Speaker Ikumi Fujita
Affiliation Institute for Protein Research, Osaka University
Title Mechanism of the nuclear oscillation in fission yeast meiosis
Poster click here to download(PDF)
Host Fumio Matsuzaki
Summary Movement of chromosomes during meiosis is crucial for homologous pairing and meiotic recombination. In fission yeast, the nucleus led by the spindle pole body (SPB) oscillates between two poles of a cylindrical cell during meiotic prophase. In this period, all telomeres are clustered at the SPB through an interaction of the telomere protein Rap1 with the meiosis-specific SPB proteins Bqt1 and Bqt2. The nuclear oscillation is driven by cytoplasmic dynein, which generates pulling forces on astral microtubules at the cell cortex. We found that the dynein regulator dynactin is essential for cortical anchoring of dynein. The dynactin subunit Arp1 temporally localized at the dynein-accumulated foci where microtubules made contact with the cell cortex. Next, we constructed a simulation model of the nuclear oscillation based on microtubule dynamic instability. A model that considers microtubule pulling and pushing forces generated oscillation similar to that of living cells. Analysis using the simulation model and time-lapse observation of living cells revealed that frequency of the nuclear oscillation is dependent on cell length. In addition, velocity of the SPB movement was increased in bqt1 mutant, in which telomeres are detached from the SPB, suggesting that the connection between chromosomes and the SPB affects velocity of the movement.
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