Seminars and Events

Past Events

Category Seminar
Date and Time 2017-12-18 16:00 - 17:00
Venue Seminar Room A7F
Speaker Shotaro Otsuka
Affiliation European Molecular Biology Laboratory (EMBL)
Title Correlative light-electron microscopy reveals how nuclear pore assembles at nano-meter resolution.
Poster click here to download(PDF)
Host Tomoya Kitajima
Summary The nuclear pore complex (NPC) is the largest non-polymeric protein complex in eukaryotic cells and spans the double membrane of the nucleus (nuclear envelope; NE) to mediate nucleocytoplasmic transport. In mammalian cells, NPCs and the NE disassemble at the beginning of mitosis and their rapid reformation during mitosis exit is essential for establishing a functional nucleus in the daughter cell. How thousands of NPCs assemble into the reforming NE had remained unclear. Recently we have established a novel correlative light and electron microscopy (CLEM) method, which allowed us to resolve for the first time the dynamic process of postmitotic NE and NPC assembly in situ in human cells at nano-meter resolution.