Retinal Regeneration

Project Leader
Masayo Takahashi (M.D., Ph.D.)

The retina has been called the “approachable part of the brain,” owing to its relatively simple structure and its location near the body surface, and for these reasons it serves as a useful and experimentally amenable model of the central nervous system. Until very recently, it was thought that in adult mammals the retina was entirely incapable of regenerating, but we now know that at least new retinal neurons can be generated after being damaged. This has opened up new hope that the ability to regenerate neurons and even to reconstitute the neural network may be retained in the adult retina. We are now exploring the exciting prospect that, by transplanting cells from outside of the retina or by regeneration from intrinsic progenitor cells, it may one day be possible to restore lost function to damaged retinas.
Our research into retinal regeneration seeks to achieve clinical applications by developing methods for inducing stem cells or embryonic stem cells to differentiate into retinal neurons and pigment epithelial cells in sufficient quantities for use in the treatment of patients suffering from conditions in which such cells have been damaged or lost. We must also ensure that such cells establish viable grafts upon transplantation and induce the reconstitution of functional neural networks. We also hope to develop means of promoting true regeneration by activating endogenous stem cells to replace cells lost to trauma or disease and thus repair damaged tissues. Access to a broad spectrum of developmental biological research information will be key to the achievement of these goals, and we appreciate the opportunities for exchange that working in the environment provided by the RIKEN CDB.

Therapeutic applications cannot be developed from basic research alone; the clinical approach – a thorough understanding of the medical condition to be treated is equally important. For conditions such as retinitis pigmentosa, even the successful transplantation of cells in animal models may not necessarily be translatable to a human clinical therapy without an understanding of the underlying genetics and possible immunological involvement. Our goal is to study retinal regeneration based on both a strong foundation in basic research and solid clinical evidence.

Select References

M Mandai, Autologous Induced Stem-Cell–Derived Retinal Cells for Macular Degeneration. New England Journal of Medicine. 2017 Mar 15. doi: 10.1056/NEJMoa1608368

Mandai M , iPSC-Derived Retina Transplants Improve Vision in rd1 End-Stage Retinal-Degeneration Mice. Stem Cell Reports. 2017 Jan 10. doi: 10.1016/j.stemcr.2016.12.008.

Sunagawa G, Takahashi M. Hypometabolism during Daily Torpor in Mice is Dominated by Reduction in the Sensitivity of the Thermoregulatory System. Scientific Reports. 2016 Nov 15. doi: 10.1038/srep37011

Sugita S, et al. Lack of T-cell response to iPS cell-derived retinal pigment epithelial cells from HLA homozygous donors. Stem Cell Reports. 2016 Sep 15. doi: 10.1016/j.stemcr.2016.08.011.

Sugita S, et al. Successful Transplantation of Retinal Pigment Epithelial Cells from MHC Homozygote iPSCs in MHC-Matched Models. Stem Cell Reports. 2016 Sep 15. doi: 10.1016/j.stemcr.2016.08.010.

Shirai H. et al. Transplantation of human embryonic stem cell-derived retinal tissue in two primate models of retinal degeneration. PNAS Plus DOI: 10.1073/pnas.1512590113

Assawachananont J, et al. Transplantation of embryonic and induced pluripotent stem cell-derived 3D retinal sheets into retinal degenerative mice. Stem Cell Reports (2014)

Kamao H, et al. Characterization of Human Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Cell Sheets Aiming for Clinical Application. Stem Cell Reports 2 1-14 (2014)

Jin Z. B, et al. Modeling retinal degeneration using patient-specific induced pluripotent stem cells. PLoS One 6.e17084 (2011)

Osakada F, et al. Toward the generation of rod and cone photoreceptors from mouse, monkey and human embryonic stem cells. Nat Biotechnol 26. 215-24 (2008)

Osakada F, et al. Wnt signaling promotes regeneration in the retina of adult mammals. J Neurosci 27. 4210-9 (2007)

Ooto S, et al. Potential for neural regeneration after neurotoxic injury in the adult mammalian retina. Proc Natl Acad Sci U S A 101. 13654-9 (2004)

Haruta M, et al. In vitro and in vivo characterization of pigment epithelial cells differentiated from primate embryonic stem cells. Invest Ophthalmol Vis Sci 45. 1020-5 (2004)

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Lab Homepage

Pilot safety study of iPSC-based intervention for wet-type AMD



iPSC-derived retinal pigment epithelium cells and cell sheet (generated for clinical use)
Mouse iPSC-derived photoreceptors are morphologically able to form synapses after transplantation into host model animals.
Human iPSCs (immunostained red:SSEA-4, blue: DAPI)